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Quantitation of DNase I sensitivity in Xenopus chromatin containing active and inactive globin, albumin and vitellogenin genes.
Felber BK
,
Gerber-Huber S
,
Meier C
,
May FE
,
Westley B
,
Weber R
,
Ryffel GU
.
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The disappearance of defined restriction fragments of the beta 1-globin, an albumin and the A1 vitellogenin gene was quantitated after DNase I digestion and expressed by a sensitivity factor defined by a mathematical model. Analysis of naked DNA showed that the gene fragments have similar but not identical sensitivity factors. DNase I digestion of chromatin revealed for the same gene fragments sensitivity factors differing over a much wilder range. This is correlated to the activity of the genes analyzed: the beta 1-globin gene fragment is more sensitive to DNase I in chromatin of erythrocytes compared to hepatocytes whereas the albumin gene fragment is more sensitive to DNase I in chromatin of hepatocytes. The A1 vitellogenin gene has the same DNase I sensitivity in both cell types. Comparing the DNase I sensitivity of the three genes in their inactive state we suggest that different chromatin conformations may exist for inactive genes.
Bellard,
Differential nuclease sensitivity of the ovalbumin and beta-globin chromatin regions in erythrocytes and oviduct cells of laying hen.
1980, Pubmed
Bellard,
Differential nuclease sensitivity of the ovalbumin and beta-globin chromatin regions in erythrocytes and oviduct cells of laying hen.
1980,
Pubmed
Felber,
Isolation and translation in vitro of four related vitellogenin mRNAs of estrogen-stimulated Xenopus laevis.
1980,
Pubmed
,
Xenbase
Kay,
Molecular cloning of cDNA sequences coding for the major alpha- and beta-globin polypeptides of adult Xenopus laevis.
1980,
Pubmed
,
Xenbase
Kuo,
DNA methylation: correlation with DNase I sensitivity of chicken ovalbumin and conalbumin chromatin.
1979,
Pubmed
Patient,
Internal organization of the major adult alpha- and beta-globin genes of X. laevis.
1980,
Pubmed
,
Xenbase
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Stalder,
Hb switching in chickens.
1980,
Pubmed
Stalder,
Tissue-specific DNA cleavages in the globin chromatin domain introduced by DNAase I.
1980,
Pubmed
Stanchfield,
An estrogen responsive primary amphibian liver cell culture system.
1978,
Pubmed
Wahli,
Electron-microscopic demonstration of terminal and internal initiation sites for cDNA synthesis on vitellogenin mRNA.
1978,
Pubmed
,
Xenbase
Wahli,
Vitellogenin in Xenopus laevis is encoded in a small family of genes.
1979,
Pubmed
,
Xenbase
Wahli,
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
1980,
Pubmed
,
Xenbase
Wahli,
Comparative analysis of the structural organization of two closely related vitellogenin genes in X. laevis.
1980,
Pubmed
,
Xenbase
Wangh,
Parenchymal cells purified from Xenopus liver and maintained in primary culture synthesize vitellogenin in response to estradiol-17 beta and serum albumin in response to dexamethasone.
1979,
Pubmed
,
Xenbase
Widmer,
Enrichment and characterization of the DNA coding for vitellogenin in Xenopus laevis.
1979,
Pubmed
,
Xenbase
Wu,
The chromatin structure of specific genes: I. Evidence for higher order domains of defined DNA sequence.
1979,
Pubmed
Wu,
The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
1979,
Pubmed
Wu,
The 5' ends of Drosophila heat shock genes in chromatin are hypersensitive to DNase I.
1980,
Pubmed
Zasloff,
Limited DNase I nicking as a probe of gene conformation.
1980,
Pubmed
