Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
J Biol Chem
2008 May 09;28319:12777-88. doi: 10.1074/jbc.M707829200.
Show Gene links
Show Anatomy links
Characterization of human SLC4A10 as an electroneutral Na/HCO3 cotransporter (NBCn2) with Cl- self-exchange activity.
Parker MD
,
Musa-Aziz R
,
Rojas JD
,
Choi I
,
Daly CM
,
Boron WF
.
???displayArticle.abstract???
The SLC4A10 gene product, commonly known as NCBE, is highly expressed in rodent brain and has been characterized by others as a Na(+)-driven Cl-HCO(3) exchanger. However, some of the earlier data are not consistent with Na(+)-driven Cl-HCO(3) exchange activity. In the present study, northern blot analysis showed that, also in humans, NCBE transcripts are predominantly expressed in brain. In some human NCBE transcripts, splice cassettes A and/or B, originally reported in rats and mice, are spliced out. In brain cDNA, we found evidence of a unique partial splice of cassette B that is predicted to produce an NCBE protein with a novel C terminus containing a protein kinase C phosphorylation site. We used pH-sensitive microelectrodes to study the molecular physiology of human NCBE expressed in Xenopus oocytes. In agreement with others we found that NCBE mediates the 4,4'-diisothiocyanato-stilbene-2,2'-disulfonic acid-sensitive, Na(+)-dependent transport of HCO(3)(-). For the first time, we demonstrated that this transport process is electroneutral. Using Cl(-)-sensitive microelectrodes positioned at the oocyte surface, we found that, unlike both human and squid Na(+)-driven Cl-HCO(3) exchangers, human NCBE does not normally couple the net influx of HCO(3)(-) to a net efflux of Cl(-). Moreover we found that that the (36)Cl efflux from NCBE-expressing oocytes, interpreted by others to be coupled to the influx of Na(+) and HCO(3)(-), actually represents a CO(2)/HCO(3)(-)-stimulated Cl(-) self-exchange not coupled to either Na(+) or net HCO(3)(-) transport. We propose to rename NCBE as the second electroneutral Na/HCO(3) cotransporter, NBCn2.
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983, Pubmed,
Xenbase
Barish,
A transient calcium-dependent chloride current in the immature Xenopus oocyte.
1983,
Pubmed
,
Xenbase
Boron,
Intracellular pH transients in squid giant axons caused by CO2, NH3, and metabolic inhibitors.
1976,
Pubmed
Boron,
Stoichiometry and ion dependencies of the intracellular-pH-regulating mechanism in squid giant axons.
1983,
Pubmed
Boron,
Intracellular pH transients in giant barnacle muscle fibers.
1977,
Pubmed
Bourgeois,
Basolateral membrane Cl(-)-, Na(+)-, and K(+)-coupled base transport mechanisms in rat MTALH.
2002,
Pubmed
Boyarsky,
pH regulation in single glomerular mesangial cells. II. Na+-dependent and -independent Cl(-)-HCO3- exchangers.
1988,
Pubmed
Chen,
Use of a new polyclonal antibody to study the distribution and glycosylation of the sodium-coupled bicarbonate transporter NCBE in rodent brain.
2008,
Pubmed
,
Xenbase
Choi,
An electroneutral sodium/bicarbonate cotransporter NBCn1 and associated sodium channel.
2000,
Pubmed
,
Xenbase
Dalmark,
Effects of halides and bicarbonate on chloride transport in human red blood cells.
1976,
Pubmed
Doughty,
Measurement of chloride flux associated with the myogenic response in rat cerebral arteries.
2001,
Pubmed
Eiden,
The vesicular amine transporter family (SLC18): amine/proton antiporters required for vesicular accumulation and regulated exocytotic secretion of monoamines and acetylcholine.
2004,
Pubmed
Giffard,
Two variants of the rat brain sodium-driven chloride bicarbonate exchanger (NCBE): developmental expression and addition of a PDZ motif.
2003,
Pubmed
Grichtchenko,
Cloning, characterization, and chromosomal mapping of a human electroneutral Na(+)-driven Cl-HCO3 exchanger.
2001,
Pubmed
,
Xenbase
Groves,
Glycophorin A facilitates the expression of human band 3-mediated anion transport in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Hübner,
Expression of the sodium-driven chloride bicarbonate exchanger NCBE during prenatal mouse development.
2004,
Pubmed
Humphreys,
Functional characterization and regulation by pH of murine AE2 anion exchanger expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Jacobs,
Mice with targeted Slc4a10 gene disruption have small brain ventricles and show reduced neuronal excitability.
2008,
Pubmed
Jarolimek,
Neurons sensitive to pH in slices of the rat ventral medulla oblongata.
1990,
Pubmed
Kietz,
pH-dependence of inhibition by H2DIDS of mouse erythroid band 3-mediated Cl- transport in Xenopus oocytes. The effect of oligonucleotide-directed replacement of Lys-558 by an Asn residue.
1991,
Pubmed
,
Xenbase
Loo,
Role of Cl- in electrogenic Na+-coupled cotransporters GAT1 and SGLT1.
2000,
Pubmed
,
Xenbase
Lu,
Reversible and irreversible interactions of DIDS with the human electrogenic Na/HCO3 cotransporter NBCe1-A: role of lysines in the KKMIK motif of TM5.
2007,
Pubmed
,
Xenbase
Odgaard,
Basolateral Na+-dependent HCO3- transporter NBCn1-mediated HCO3- influx in rat medullary thick ascending limb.
2004,
Pubmed
Okubo,
Red blood cell band 3. Lysine 539 and lysine 851 react with the same H2DIDS (4,4'-diisothiocyanodihydrostilbene-2,2'-disulfonic acid) molecule.
1994,
Pubmed
Pasternack,
Proton modulation of functionally distinct GABAA receptors in acutely isolated pyramidal neurons of rat hippocampus.
1996,
Pubmed
Piermarini,
Cloning and characterization of an electrogenic Na/HCO3- cotransporter from the squid giant fiber lobe.
2007,
Pubmed
,
Xenbase
Praetorius,
Distribution of sodium transporters and aquaporin-1 in the human choroid plexus.
2006,
Pubmed
Praetorius,
A SCL4A10 gene product maps selectively to the basolateral plasma membrane of choroid plexus epithelial cells.
2004,
Pubmed
Pushkin,
Cloning, tissue distribution, genomic organization, and functional characterization of NBC3, a new member of the sodium bicarbonate cotransporter family.
1999,
Pubmed
,
Xenbase
Ransom,
Glial modulation of neural excitability mediated by extracellular pH: a hypothesis.
1992,
Pubmed
Romero,
The SLC4 family of HCO 3 - transporters.
2004,
Pubmed
Romero,
Cloning and functional expression of rNBC, an electrogenic Na(+)-HCO3- cotransporter from rat kidney.
1998,
Pubmed
,
Xenbase
Roos,
Intracellular pH.
1981,
Pubmed
Russell,
Role of choloride transport in regulation of intracellular pH.
1976,
Pubmed
Schwiening,
Regulation of intracellular pH in pyramidal neurones from the rat hippocampus by Na(+)-dependent Cl(-)-HCO3- exchange.
1994,
Pubmed
Sebat,
Strong association of de novo copy number mutations with autism.
2007,
Pubmed
Spray,
Gap junctional conductance is a simple and sensitive function of intracellular pH.
1981,
Pubmed
Thomas,
The role of bicarbonate, chloride and sodium ions in the regulation of intracellular pH in snail neurones.
1977,
Pubmed
Toye,
The human NBCe1-A mutant R881C, associated with proximal renal tubular acidosis, retains function but is mistargeted in polarized renal epithelia.
2006,
Pubmed
,
Xenbase
Virkki,
Cloning of a Na+-driven Cl/HCO3 exchanger from squid giant fiber lobe.
2003,
Pubmed
,
Xenbase
Virkki,
Functional characterization of human NBC4 as an electrogenic Na+-HCO cotransporter (NBCe2).
2002,
Pubmed
,
Xenbase
Waldmann,
A proton-gated cation channel involved in acid-sensing.
1997,
Pubmed
,
Xenbase
Wang,
The Na+-driven Cl-/HCO3- exchanger. Cloning, tissue distribution, and functional characterization.
2000,
Pubmed
,
Xenbase
