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Dev Neurobiol 2014 Sep 01;749:894-906. doi: 10.1002/dneu.22173.
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Transcription factor AP2 epsilon (Tfap2e) regulates neural crest specification in Xenopus.

Hong CS , Devotta A , Lee YH , Park BY , Saint-Jeannet JP .

Transcription factors Pax3 and Zic1 are two important regulators of cell fate decision at the neural plate border, where they act synergistically to promote neural crest (NC) formation. To understand the role of these factors in NC development, we performed a microarray analysis to identify downstream targets of Pax3 and Zic1 in Xenopus embryos. Among the genes identified was a member of transcription factor activator protein 2 (Tfap2) family, Tfap2 epsilon (Tfap2e). Tfap2e is first expressed at early neurula stage in NC progenitors and Rohon-Beard sensory neurons, and persists in a subset of migrating cranial NC cells as they populate the pharyngeal arches. This is in contrast to other species in which Tfap2e is not detected in the early NC lineage. Tfap2e morpholino-mediated knockdown results in a loss of NC progenitors and an expansion of the neural plate. Tfap2e is also sufficient to activate NC-specific genes in animal cap explants, and gain-of-function experiments in the whole embryo indicate that Tfap2e can promote NC formation. We propose that Tfap2e is a novel player in the gene regulatory network controlling NC specification in Xenopus downstream of Pax3 and Zic1.

PubMed ID: 24616412
PMC ID: PMC4107115
Article link: Dev Neurobiol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: astl2c astl3a.1 eef1a2 fgf8 msx1 npb pax3 runx1 snai2 sox10 sox2 sox8 tbx2 tfap2a tfap2e wnt8a zic1
Morpholinos: tfap2e MO1

Article Images: [+] show captions
References [+] :
Aoki, Sox10 regulates the development of neural crest-derived melanocytes in Xenopus. 2003, Pubmed, Xenbase