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J Cell Biol 2019 Aug 05;2188:2659-2676. doi: 10.1083/jcb.201811147.
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Developmentally regulated GTP-binding protein 1 modulates ciliogenesis via an interaction with Dishevelled.

Lee M , Hwang YS , Yoon J , Sun J , Harned A , Nagashima K , Daar IO .

Cilia are critical for proper embryonic development and maintaining homeostasis. Although extensively studied, there are still significant gaps regarding the proteins involved in regulating ciliogenesis. Using the Xenopus laevis embryo, we show that Dishevelled (Dvl), a key Wnt signaling scaffold that is critical to proper ciliogenesis, interacts with Drg1 (developmentally regulated GTP-binding protein 1). The loss of Drg1 or disruption of the interaction with Dvl reduces the length and number of cilia and displays defects in basal body migration and docking to the apical surface of multiciliated cells (MCCs). Moreover, Drg1 morphants display abnormal rotational polarity of basal bodies and a decrease in apical actin and RhoA activity that can be attributed to disruption of the protein complex between Dvl and Daam1, as well as between Daam1 and RhoA. These results support the concept that the Drg1-Dvl interaction regulates apical actin polymerization and stability in MCCs. Thus, Drg1 is a newly identified partner of Dvl in regulating ciliogenesis.

PubMed ID: 31270137
PMC ID: PMC6683737
Article link: J Cell Biol

Species referenced: Xenopus laevis
Genes referenced: daam1 drg1 drg2 dvl1 dvl2 dvl3 fmn1 isyna1 limk1 mcc rho rhoa tal1
Morpholinos: daam1 MO1 drg1 MO1 drg1 MO2 dvl1 MO1 dvl3 MO1

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References [+] :
Bernstein, ADF/cofilin: a functional node in cell biology. 2010, Pubmed