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Nat Commun 2020 Jul 13;111:3491. doi: 10.1038/s41467-020-17238-w.
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Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription.

Oikawa M , Simeone A , Hormanseder E , Teperek M , Gaggioli V , O'Doherty A , Falk E , Sporniak M , D'Santos C , Franklin VNR , Kishore K , Bradshaw CR , Keane D , Freour T , David L , Grzybowski AT , Ruthenburg AJ , Gurdon J , Jullien J .

Sperm contributes genetic and epigenetic information to the embryo to efficiently support development. However, the mechanism underlying such developmental competence remains elusive. Here, we investigated whether all sperm cells have a common epigenetic configuration that primes transcriptional program for embryonic development. Using calibrated ChIP-seq, we show that remodelling of histones during spermiogenesis results in the retention of methylated histone H3 at the same genomic location in most sperm cell. This homogeneously methylated fraction of histone H3 in the sperm genome is maintained during early embryonic replication. Such methylated histone fraction resisting post-fertilisation reprogramming marks developmental genes whose expression is perturbed upon experimental reduction of histone methylation. A similar homogeneously methylated histone H3 fraction is detected in human sperm. Altogether, we uncover a conserved mechanism of paternal epigenetic information transmission to the embryo through the homogeneous retention of methylated histone in a sperm cells population.

PubMed ID: 32661239
PMC ID: PMC7359334
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus
Genes referenced: ascl2 atf1 bmi1 cbx3 cfap52 cpeb1 elf3 foxq1 h2bc21 hmga2 hmgb1 hmgb2 hmgb3 hmgn2 meis1 meis2 nfya nfyb nhlh1 nr4a2 osr2 pam sox7 sp1 spdef spib stat1 tbx3 tcf12 tcf3 wdr5 zbtb33 zic3 znf143 znf281
Antibodies: H3C1 Ab10 Hist1H3A Ab2 Histone H2B Ab10 Histone H4 Ab11 Hmgb1 Ab1 Tri-Methyl H3f3a Ab19

GEO Series: GSE125982: NCBI

Article Images: [+] show captions
References [+] :
Akkers, Chromatin immunoprecipitation analysis of Xenopus embryos. 2012, Pubmed, Xenbase