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Int J Mol Sci 2022 Oct 11;2320:. doi: 10.3390/ijms232012078.
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The Flavonol Quercitrin Hinders GSK3 Activity and Potentiates the Wnt/β-Catenin Signaling Pathway.

Predes D , Maia LA , Matias I , Araujo HPM , Soares C , Barros-Aragão FGQ , Oliveira LFS , Reis RR , Amado NG , Simas ABC , Mendes FA , Gomes FCA , Figueiredo CP , Abreu JG .

The Wnt/β-catenin signaling pathway dictates cell proliferation and differentiation during embryonic development and tissue homeostasis. Its deregulation is associated with many pathological conditions, including neurodegenerative disease, frequently downregulated. The lack of efficient treatment for these diseases, including Alzheimer's disease (AD), makes Wnt signaling an attractive target for therapies. Interestingly, novel Wnt signaling activating compounds are less frequently described than inhibitors, turning the quest for novel positive modulators even more appealing. In that sense, natural compounds are an outstanding source of potential drug leads. Here, we combine different experimental models, cell-based approaches, neuronal culture assays, and rodent behavior tests with Xenopus laevis phenotypic analysis to characterize quercitrin, a natural compound, as a novel Wnt signaling potentiator. We find that quercitrin potentiates the signaling in a concentration-dependent manner and increases the occurrence of the Xenopus secondary axis phenotype mediated by Xwnt8 injection. Using a GSK3 biosensor, we describe that quercitrin impairs GSK3 activity and increases phosphorylated GSK3β S9 levels. Treatment with XAV939, an inhibitor downstream of GSK3, impairs the quercitrin-mediated effect. Next, we show that quercitrin potentiates the Wnt3a-synaptogenic effect in hippocampal neurons in culture, which is blocked by XAV939. Quercitrin treatment also rescues the hippocampal synapse loss induced by intracerebroventricular injection of amyloid-β oligomers (AβO) in mice. Finally, quercitrin rescues AβO-mediated memory impairment, which is prevented by XAV939. Thus, our study uncovers a novel function for quercitrin as a Wnt/β-catenin signaling potentiator, describes its mechanism of action, and opens new avenues for AD treatments.

PubMed ID: 36292931
PMC ID: PMC9602613
Article link: Int J Mol Sci

Species referenced: Xenopus laevis
Genes referenced: gsk3b wnt3a wnt8a
GO keywords: Wnt signaling pathway

Disease Ontology terms: Alzheimer's disease [+]
Phenotypes: Xla Wt + wnt8a (Fig. 2 abcgh) [+]

Article Images: [+] show captions
References [+] :
Alvarez, Wnt-3a overcomes beta-amyloid toxicity in rat hippocampal neurons. 2004, Pubmed