Fig. 5. Sulf1 is required for the normal distribution of Shh protein in vivo. (AâD) Immunostaining embryos unilaterally injected with a control morpholino with 5E1reveals Shh protein in the notochord (NC) and the neural tube (NT), with highest levels in the floor plate (FP). In these control embryos the level of Shh protein in the neural tube drops off sharply away from the FP and is not detected within the dorsal neural tube (C). (EâH) Unilateral knockdown of Sulf1 in vivo leads to a change in the distribution of Shh protein on the injected side (*) where there is reduced Shh protein detected which is detected much further dorsally in a more diffuse pattern than in controls (compare G with C). (I) Quantification of the immunohistochemistry reveals a marked change in the diffusion of Shh away from its source in the absence of Sulf1 expression. The level of Shh in controls embryos (blue) immediately adjacent to Shh producing cells is high; this drops to almost zero within ~20 μm. In the absence of Sulf1(red), the level of ligand adjacent to producing cells is lower than in controls, but this level remains higher much further from the source, only dropping to zero ~50 μm from the source cells. Graph represents average grey level across the width of the neural tube on the injected side. Area measured for quantification shown. Mean values from a number of samples are shown (CMO n=5, AMO n=7).
Image published in: Ramsbottom SA et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| shh | hedgehog, vhh-1, Xhh, X-SHH, xshh | X. tropicalis | Sometime during NF stage 23 to NF stage 35 and 36 | notochord floor plate neural tube |
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