Figure 1. (A) All subunits of the heterotrimeric motor protein, kinesin II, are present on melanosomes. Western blots of cell extracts and purified melanosomes probed with antibodies against the motor subunits of kinesin II. Lanes C are cell extracts, and lanes M are purified melanosomes. The first two lanes were probed with K2.4 to detect the 85-kD motor subunit, the following two lanes were probed with a polyclonal anti-Xklp3 to detect the 95-kD motor subunit, and the last two lanes were probed with a polyclonal anti–sea urchin KAP 115 to detect the 115-kD accessory subunit. Notice that all the subunits are enriched in the melanosome fraction. (B) The overexpressed EGFP-headless Xklp3 protein forms a complex with the endogenous 85-kD motor subunit. Melanophore extracts were prepared from cells transfected with pEGFP-C1 or pEGFP-headless Xklp3, immunoprecipitated with an anti-GFP antibody, and probed with K2.4 to detect the 85-kD motor subunit. Shown are Western blots from both immunoprecipitates. The 85-kD subunit coimmunoprecipitates with EGFP-headless Xklp3 (lane 1), but not with EGFP alone (lane 2).
Image published in: Tuma MC et al. (1998)
Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution-NonCommercial-ShareAlike license
Permanent Image Page
Printer Friendly View
XB-IMG-117735