Larger Image Figure 8. . Thermodynamic analysis of block of wild-type and double mutant (E224G + E299S) IRK1 channels by a short and a long alkyl-bis-amine. The four corners of the thermodynamic cycle are Kds (0 mV) (mean ± SEM; n = 5–8) of wild-type and mutant channels for bis-C4 and bis-C9. The numbers around the cycle are the ratios of adjacent Kds. Ω = (wtKdbis-C4 × E224G+E299SKdbis-C9)/(E224G+E299SKdbis-C4 × wtKdbis-C9). Image published in: Guo D et al. (2003) Copyright © 2003, The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Permanent Image Page Printer Friendly View XB-IMG-120273