Figure 7. Effect of mutations at residue C166 on the coupling of Kir6.2 to SUR1. (A and B) Macroscopic currents recorded from inside-out patches in response to a series of voltage ramps from −110 to +100 mV. Oocytes were injected with mRNAs encoding Kir6.2 + SUR1 (A) or Kir6.2-C166S + SUR1 (B). MgADP, MgATP, diazoxide (DZ), or tolbutamide (TB) were added to the internal solution as indicated by the bars. (C) Mean macroscopic slope conductance recorded in the presence of the agents indicated (G), expressed as a fraction of the mean (Gc) of that obtained in control solution (no additions) before and after exposure to the test compound(s). The dashed line indicates the current level in the absence of the test compound. Oocytes were injected with mRNAs encoding Kir6.2 + SUR1 (white bars), Kir6.2-C166S + SUR1 (black bars), or Kir6.2ΔC26-C166V + SUR1 (hatched bars). The number of oocytes is given above the bars. *P < 0.05, **P < 0.01 against Kir6.2 + SUR1.
Image published in: Trapp S et al. (1998)
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