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Larger Image Figure 9. Wavelength dependence of UV sensitivity of CNG channels. (A) Action spectrum of UV effect compared with wavelength dependence of tryptophan and tyrosine absorption. The UV sensitivity at each excitation wavelength λ, defined as the reciprocal of D1/2(λ), was obtained from the fits in Fig. 8. Also shown is the UV sensitivity at 266 nm, obtained from the results in Fig. 7 B. The UV sensitivity (•, mean ± SEM), normalized to a value of 1.0 at 270 nm, is plotted as a function of wavelength on semilogarithmic coordinates. The predicted action spectra for photochemical modification of tryptophan (solid line) and tyrosine (dotted line) targets were calculated from their absorption spectra (Fig. 3) using and were normalized to a value of 1 at 270 nm. (B) Wavelength dependence of slope factor. The slope factors from the fits to the results in Fig. 7 B and 8 are plotted as a function of wavelength on linear coordinates. The experimental points (•) are mean ± SD. Dashed lines show the range of the estimated slope factors for excitation wavelengths between 260 and 310 nm. Image published in: Middendorf TR et al. (2000) © 2000 The Rockefeller University Press. Creative Commons Attribution-NonCommercial-ShareAlike license Permanent Image Page Printer Friendly View XB-IMG-121124 |