Figure 3. . Hydrolyzable ATP prevented SK channel run-down in inside-out patches. (A) In the absence of Mg-ATP, channel activity rapidly decreased in the Ca2+/Mg2+-free Ringer's solution. 0.5 mM Mg-ATP reactivated the run-down channel. (B) SK channel activity recorded from an inside-out patch shows run-down in the presence of ATPγS (0.5 mM)-containing, Ca2+-free bath solution. The SK channel activity could be reactivated by 0.5 mM Mg-ATP in a Ca2+-free Ringer solution. The time to run-down was similar to those shown in Figs. 1 and 3 A. C = channel-closed state. −Vpipette = −40 mV.
Image published in: Lu M et al. (2002)
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