Figure 1. . Deletions of COOH-terminal residues in Shaker. (A) Sequence alignment of S6 and initial part of the COOH terminus for four voltage-gated K+ channels (the arrow indicates the last conserved residue in the region). (B–G) Current records from oocytes injected with RNA encoding Shaker or mutants that lack the indicated residues in the region under the horizontal bar in A. Dotted lines identify the zero current level. (H) G-V curves for Shaker and the HRE deletion mutant; the data points represent mean currents (± SEM, n = 15 and 6). The fitted curves correspond to the Boltzmann function, yielding V1/2 = −32.8 ± 0.3 mV and valence (Z) = 3.9 ± 0.3 for Shaker, and V1/2 = 6.8 ± 1.3 mV (n = 6) and Z = 1.1 ± 0.1 for the HRE deletion mutant.
Image published in: Lu Z et al. (2002)
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