Figure 5. . Macroscopic currents recorded in the presence of the indicated concentrations of spermine from excised patches of HEK293T cells expressing wild-type or mutant Kir2.1. Note the difference in concentration ranges used for wild-type and S165L (0.01, 0.1, and 1 μM), D172N and D172N & S165L (0.1, 1, and 10 μM), and D172N & E224G & E299Q and D172N & E224G & E299Q & S165L (1, 10, and 100 μM) channels. K+o and K+i were 20 and 140 mM, respectively. The holding potential was −50 mV, and step pulses from 40 to −120 mV were applied in 10-mV decrements.
Image published in: Fujiwara Y and Kubo Y (2002)
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