Figure 1. . No time dependence in the blockage of BK currents by C10. (A) Time-dependent block of Shaker K+ channels by C10. Macroscopic K+ currents were recorded from an inside-out patch from an oocyte expressing Shaker B Δ6-46 channels. Under voltage clamp, currents were elicited by depolarization of the membrane potential to 60 mV from a holding potential at −120 mV. Application of 2 μM (dark gray trace) and 10 μM C10 (light gray trace) resulted in reductions of current from the control level (black trace). (B) Macroscopic K+ currents carried by BK channels and their responses to C10. Currents were recorded under voltage clamp from an inside-out patch from an oocyte expressing mslo BK channels. Currents were elicited by depolarizations of the membrane potential to 40, 80, and 120 mV from a holding potential at −80 mV. Currents before (black) and after the application of 10 μM (dark gray) and 100 μM C10 (light gray) are shown with the same scale in a, b, and c, respectively. Current traces in this and all other figures represent the average of 4–8 consecutive series. The dashed lines indicate the zero current level in this and all other figures with macroscopic current traces.
Image published in: Li W and Aldrich RW (2004)
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