Figure 8. . Ca2+ binding for a series of mutant fusion proteins. (A) 45Ca2+-band densities from overlay assays are plotted as a percentage of wild-type band density for each of ten GST-mSlo207 fusion proteins. (B) 45Ca2+-band density is plotted for each mutant fusion protein as a function of the change in net charge each mutation brings about. Open circles indicate mutations outside the central acidic region of the Ca2+ bowl (896–901). Closed circles indicate mutations inside this central region.
Image published in: Bao L et al. (2004)
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