Figure 2. . Macroscopic I-V relationship for different concentration of spermine. (A) Representative current traces recorded from inside-out patch in the presence of 0 (control), 1, and 100 μM spermine. The dotted line indicates the zero current level. Voltage protocol is shown at the bottom. (B) Steady-state I-V curves for control (), and 1 μM (○), 10 μM (□), 100 μM (▵) and 1 mM (▿) spermine. (C) The ratio of the steady-state current (I) in the presence of spermine to that in its absence (Ictl) plotted against voltage, for the spermine concentrations in B (with corresponding symbols). (D) Comparison of the concentration dependence of the spermine block at −100 mV (•) and −20 mV (▪), respectively, from the experimental data in C. Solid lines are best fits to a Hill equation: I/Ictl = 1/(1 + ([spm]/Kd)H), where Kd is spermine concentration causing half-maximal block and H is the Hill coefficient. The values of H at −100 and −20 mV are as indicated.
Image published in: Xie LH et al. (2002)
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