Figure 2. Gating and selectivity of ClC-Kb-R538P.Two-electrode voltage clamp recordings of (A) wild type and (B) R538P ClC-Kb channels. Currents are in response to a 200-ms prepulse to +60 mV followed by 500-ms test pulses ranging from +60 mV to −140 mV in −20 mV decrements. (C) Steady-state currents at the different voltages were normalized to the current value at +60 mV for wild type (filled squares) and R538P (open squares). Error bars are s.e.m. with n = 8 (WT) and n = 6 (R538P). Error bars smaller than the symbols are not shown. Representative steady state I–V curves for (D) wild type and (E) R538P channels in the presence of chloride (filled squares), bromide (open squares), or iodide (open diamonds). (F) Permeability ratios relative to chloride for wild type (filled bars) and R538P (open bars). The bromide/chloride permeability ratio of R538P is statistically different from that of wild type (p<0.0005, n = 5).
Image published in: Martinez GQ and Maduke M (2008)
Martinez, Maduke. Creative Commons Attribution license
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