Figure 2. Ethidium does not activate the intra-S phase checkpoint. (A) Replication time-courses in the control extract (Ctl) or in the presence of 100 µM ethidium bromide (EB), 2 µg/ml aphidicolin (Aphi), with or without 5 mM caffeine (Caff). (B) Western blots of nuclei purified at 60 min from control extracts (Ctl), or extracts containing 0.5 μg/ml bleomycin (Bleo), 50 ng/µl double-stranded poly-dA/poly-dT oligonucleotides (pApT), 100 µg/ml aphidicolin (Aphi), 100 µg/ml aphidicolin and 5 mM caffeine (A + C), or 100 µM ethidium bromide (EB). Non-specific bands are shown as loading control. (C) Analysis of H2A.X phosphorylation in 0.5 μg/ml bleomycin (Bleo) or 100 μM ethidium bromide (EB) treated extract by immunofluorescence, at indicated time-points (bar, 10 μm).
Image published in: Krasinska L and Fisher D (2009)
© 2009 The Author(s). Creative Commons Attribution-NonCommercial license
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