Figure 4. GST-XRCC1-BRCT1 overexpressed in HeLa cells triggers PAR synthesis and is poly(ADPribosyl)ated. HeLa cells overexpressing GST-hXRCC1-BRCT1 (amino acids 282–428, lanes 1–3), GST-hXRCC1-BRCT2 (amino acids 427–633, lanes 4–6) or GST (lanes 7, 8) were either untreated (lanes 1, 4 and 7) or treated with 2.5 mM MMS for 30 min (lanes 2, 5 and 8). Where indicated, cells were pre-incubated for 2 h with 100 nM of the PARP inhibitor Ku-0058948 (lanes 3 and 6). (A) Inputs corresponding to 2% of each lysates. Proteins were analyzed by GST pull down and western blot (B) using successively the anti-PAR, anti-PARP-1 and anti-GST antibodies.
Image published in: Lévy N et al. (2009)
© 2009 The Author(s). Creative Commons Attribution-NonCommercial license
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