Figure 4. Western blots of WT (Cx32*Cx43E1) and mutant (A43C) membrane-inserted hemichannels. + and − symbols at the bottom of each panel denote treatment of the sample with or without either 50 mM DTT or 10 mM TPEN before SDS-electrophoresis through 5–40% gradient polyacrylamide gels. The position of pre-stained molecular weight standards (Thermo Fisher Scientific) are presented as bars on the right side of the figure. The band with molecular weight ∼50 kD corresponds to a connexin dimer, whereas the monomer has an electrophoretic mobility comparable to a molecular weight standard of ∼27 kD. Only treatment with DTT reduces the dimer to the monomeric connexin form.
Image published in: Tang Q et al. (2009)
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