Figure 7. In vitro transport assays of NLS- and M9-linked fluorescent BSA conjugates after preload of the NPCs by P-rC.NPCs (yellow) were visualized by indirect immune fluorescence using mAb414. P-rC (cyan): Indirect capsid immune fluorescence using anti capsid antibodies. The panel show increasing signal strength related to the amounts of capsids subjected to the cells. NLS (red): fluorescence of Alexa594 NLS-BSA. M9 (blue): Fluorescence of Alexa647 M9-BSA. While the NLS-import rapidly decreased with increasing preload of the NPCs with P-rC, the M9-import was less affected. A. Negative control at 4°C without capsid preload. B. Positive control at 37°C without capsid preload. C. Preload of the nuclei with 25 ng P-rC, D. 100 ng P-rC, E. 400 ng P-rC, F. 1600 ng P-rC, G. 6400 ng P-rC, H. 12800 ng P-rC. All pictures were taken at the same magnification. Space bar: 20 µm.
Image published in: Schmitz A et al. (2010)
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