Figure 1. Both zebrafish eaf1 and eaf2/u19 genes are downstream factors of zebrafish Wnt4a.(A) Zebrafish embryos were injected with Wnt4-MO and wnt4a mRNA and the levels of zebrafish eaf1 mRNA determined by whole-mount in situ hybridization at 12-somite stage. (B) Zebrafish embryos were injected with Wnt4-MO and wnt4a mRNA and the levels of zebrafish eaf/u19 mRNA determined by whole-mount in situ hybridization. 12s, 12 somites. (C) Semi-quantitative RT-PCR analysis of eaf1 expression in zebrafish embryos (12 somites) injected with Wnt4a-MO and wnt4a mRNA. (D) Semi-quantitative RT-PCR analysis of eaf2/u19 expression in zebrafish embryos (12 somites) injected with Wnt4a-MO and wnt4a mRNA.
Image published in: Wan X et al. (2010)
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