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Larger Image Fig. 6. S-type and R-type anion currents in A. thaliana guard cell protoplasts. (A, B) Whole-cell S-type anion currents in response to a high extracellular calcium concentration (40âmM CaCl2 in the bath solution). (A) Representative traces of calcium-activated S-type anion currents. (B) Currentâvoltage relationships of the wild type (nâ=â6), atalmt12-1 (nâ=â6) and cpk6-1 (nâ=â3). (CâE) Whole-cell S-type anion currents with 10âμM (C, D) or 50âμM (E) ABA in the bath solution. (C) Representative traces of S-type anion currents. (D) Currentâvoltage relationships of the wild type (nâ=â5) and atalmt12-1 (nâ=â7). (E) Currentâvoltage relationship of the wild type and atalmt12-1 (nâ=â2). (F) Representative traces of R-type anion currents in the wild-type and atalmt12-1. (G) Average peak R-type anion channel current in the wild type (nâ=â4) and atalmt12-1 (nâ=â3). All data are the meanâ±âSEM. Significant differences (Studentâs t-test) were not observed between the wild type and atalmt12-1 in both the S-type anion current (E; Pâ=â0.587 at â115âmV) and the R-type anion current (F; Pâ=â0.348 at negative peak values). Permanent Image Page Printer Friendly View XB-IMG-124778 |