Figure 5. Pol-δ, Pol-ε, and PCNA are required for the accumulation of small nascent DNAs. (A–F) Sperm chromatin (2,500 nuclei/µl) was added to mock-, Pol-δ–, Pol-ε–, or PCNA-depleted LSE with or without 15 µM aphidicolin (n = 3). (A and D) Depletion was verified by immunoblotting. (B and E) Replication was monitored in the absence of aphidicolin as in Fig. 1 D. (C) Synthesis of small nascent DNAs was monitored in aphidicolin-treated extracts and quantitated as in Fig. 1 B. (F) Small nascent DNAs were monitored as in C.
Image published in: Van C et al. (2010)
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