Figure 5. Model for Nup107-160 and γ-TuRC regulation at kinetochoresStep 1: Nup107-160 (dark blue) and active γ-TuRC (purple) bind to unattached kinetochores. We postulate that a transport cargo (plum) may be released in the high Ran-GTP environment that maintains γ-TuRC in an active state.Step 2: Nup107-160 and γ-TuRC nucleate MTs (green lines) at kinetochores, promoting the assembly of k-fibers.Step 3: RanGAP1 (magenta) is recruited along k-fibers. Its recruitment lowers Ran-GTP levels near kinetochores. The lower Ran-GTP levels in turn allow Importin-b or another import receptor to bind the cargo protein.Step 4: After the cargo molecule is sequestered, γ-TuRC becomes inactive (pale lilac), but k-fibers remain stable.
Image published in: Mishra RK et al. (2010)
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