Figure 6. G39A substitution weakens Gadd45a association with nuclear speckles.A,B IF microscopy comparison of nuclear pattern after detergent extraction of EGFP-Gadd45a wild type (A) and EGFP-G39A mutant (B). Experiments were done essentially as in Figure 3A. C, Statistical analysis of immunofluorescence patterns as in Figure 3F. D, Western blot analysis of RKO cells expressing EGFP-Gadd45a wild type (wt) or EGFP-G39A mutant harvested without or after detergent extraction. Scale bar, 4 µm.
Image published in: Sytnikova YA et al. (2011)
Sytnikova et al. Creative Commons Attribution license
Permanent Image Page
Printer Friendly View
XB-IMG-125652