Figure 1. Set-up and circuit model.A) Changes in RF membrane impedance (|ÎZRF|) during TEVC were measured by passing RF current from an electrode surrounding the meridian of the cell (black) to a ground electrode (media, above the cell). Contour lines and colors of the saggital cross-section of a cell in the recording chamber, shown here, illustrate the general spatial distribution of the RF electric potential expected based on the Maxwell equations for a passive cell under axisymmetric conditions (Ï/4 phase shown). B) A circuit model of the chamber including the shunt resistance (Rs,), membrane impedance (Zm), intracellular resistance (Ri), and electrode double layer (Zdl). C) Using the circuit model, the frequency-dependent RF impedance would change (Î|ZRF|) with an increase or decrease in membrane capacitance â a change that would be most easily detectable at frequency Ï* where the maxima of the |ÎZRF| occurs. The present study reports changes in RF impedance |ÎZRF| evoked by TEVC step changes in membrane potential.
Image published in: Dharia S and Rabbitt RD (2011)
Dharia, Rabbitt. Creative Commons Attribution license
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