Figure 2. Temporally Resolved RF Measurements.A) RF impedance changes (|ÎZRF|) measured during TEVC relative to the impedance at holding potential (â90 mV) in control oocytes, expressing endogenous proteins only (nâ=â10, left column), and ShB-IR expressing oocytes (nâ=â9, right column). ShB-IR expressing oocytes elicited a membrane-potential-dependent (Vm*) RF response different than control oocytes. RF impedance changes were analyzed in two regions; the RF response during the onset of voltage-step (o, average |ÎZRF|o 0â1 ms after voltage step, dVm*/dt > 0) and the RF response after membrane potential achieved its command (steady-state) level (s, average |ÎZRF|s 5â35 ms after voltage step, dVm*/dt â 0). B) TEVC current measurements were used to verify ion-channel expression and responses (leak current subtracted, capacitive transient unsubtracted) to C) whole-cell voltage-clamp.
Image published in: Dharia S and Rabbitt RD (2011)
Dharia, Rabbitt. Creative Commons Attribution license
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