Figure 4. ULD-SDD restricts IKKβ specificity while ULD is required for catalytic activitya, b,Pulldown of hIKKβ constructs using GST-IκBα constructs, showing the reciprocal interaction between ULD-SDD of IKKβ and C-terminal region of IκBα containing ankyrin repeats and PEST region. c, d, Measurement of Km and relative Vmax of IKKβ against full-length IκBα (c) and the N-terminal region of IκBα (1–54) (d). a.u.: arbitrary unit. e, Kinase assay of purified hIKKβ proteins against IκBα, its S32A/S36A mutant (AA), or its PEST-deletion construct (ΔPEST, 1–282) using [γ-32P]ATP. f, Kinase assay of purified hIKKβ proteins using antibody against IκBα phosphorylated at S32 and S36. g, A schematic model showing that the interaction between SDD of IKKβ and C-terminal region of IκBα may position the N-terminal cognate phosphorylation sites of IκBα to the active site of IKKβ.
Image published in: Xu G et al. (2011)
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