Figure 1. Uncoupling mutants I384N and F484G. (A) Location of the uncoupled mutants in the Kv1.2 crystal structure (Long et al., 2005a). Shown is a single subunit. I384 is located on the S4-S5 linker, and F481 and F484 are located on the C-terminal S6, which anneals to the S4-S5 linker. (right) Magnification of the interaction site. (B) Gating currents of ShakerIR-W434F, ShakerIR-W434F-I384N, and ShakerIR-W434F-F484G in response to a series of depolarizing pulses from a holding potential of Vhold = −90 mV to a potential of −120 to 60 mV in steps of 10 mV for 50 ms. (C) QVON and QVOFF of ShakerIR-W434F, ShakerIR-W434F-I384N, and ShakerIR-W434F-F484G. (D) Fluorescence voltage (FV) and conductance voltage (GV) relations for ShakerIR-A359C, ShakerIR-A359C-I384N, and ShakerIR-A359C-F484G elicited from a series of depolarizing pulses from a holding potential of Vhold = −90 mV to a potential of −120 to 60 mV/200 mV in steps of 10 mV for a duration of 50 ms. QV and GV are far separated in the uncoupled mutants I384N and F484G. (C and D) Data are shown as mean ± SD.
Image published in: Haddad GA and Blunck R (2011)
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