Figure 6. An example of making multiple mutations across a wide region. (A) A fragment of the human nAChR α2 subunit cDNA with 8 arginine (R, in bold) codons (top) to be substituted by glutamine (Q) codons (bottom). Mutated nucleotides are indicated in red. (B) Actual forward (45-base) and reverse (46-base) primers with 16 bp overlapping in their 5' ends.
Image published in: Li C et al. (2011)
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