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Larger Image Figure 2. (A) SOFAST-HMQC spectrum of APETx2_wt (black) overlaid with that of APETx2_1–39 (red). A crosspeak is present for each H-N bond (i.e., for each non-proline residue), including non-labile sidechain NH groups (Trp Nε-Hε, Asn Nδ-Hδ2, Gln Nε-Hε and Arg Nε-Hε). (B) Backbone 15N chemical shifts for APETx2_wt (black), APETx2_1–39 (red) and APETx2_3–42 (cyan). Image published in: Jensen JE et al. (2012) © 2012 by the authors; licensee MDPI, Basel, Switzerland. Creative Commons Attribution license Permanent Image Page Printer Friendly View XB-IMG-127368 |