Larger Image Fig. 4. Forms of Ngn3 lacking lysines are stabilised by Mg132.Degradation assays at a fixed point of 90†minutes were performed in interphase (A) and mitotic (B) Xenopus egg extract using 35S-IVT Ngn3 and mutants thereof, as labelled, in either the presence or absence of 100†µM Mg132 proteasome inhibitor. Samples were run on SDS-PAGE gels, analysed by autoradiography (A,B). Image published in: Roark R et al. (2012) © 2012. Creative Commons Attribution-NonCommercial-ShareAlike license Permanent Image Page Printer Friendly View XB-IMG-128028