Figure 3. MTSEA modification of E109C attenuates Cd2+ stabilization of the loop-gate closed state. (A) A segment of a continuous current trace evoked by a train of alternating voltage polarizations between −10 mV (15 s duration) and −70 mV (5 s duration). The colored solid bars indicate the onset and duration of specified treatments. Capacitive transients were reduced but not fully eliminated by 100× data reduction in Clampfit. (B) Expansion of steady-state current traces shown in A as indicated by arrows. Peak currents obtained at steady-state at −70 mV are marked by arrows.
Image published in: Kwon T et al. (2013)
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