Figure 3. a) Analysis of cross-linking G-quadruplex in the presence of complementary strand of Pu27 DNA (Pu27-c) in 10 mM Tris/HCl buffer at pH 7.0 and 10 mM KCl by 20% polyacrylamide gel. Lane 1: labeled Pu27 DNA (10 pmol) treated at 37°C for 1 h in 10 mM KCl; lane 2: control lane without tyrosinase and compound 1; lane 3: control lane only with tyrosinase and without the compound 1; lane 4: control lane only with compound 1 and without the tyrosinase; lane 5: compound 1 (2 mM) incubation with labeled Pu27 DNA (10 pmol) and tyrosinase (40 Uints) in the presence of excess of Pu27-c (20 pmol). b) CD spectrum for examining the structural features of the cross-linked complex which was extracted from gel. c) CD melting curves of the cross-linked complex as monitored by the CD intensity at 265 nm. d) MALDI-TOF MS spectrum of the cross-linked complex, calcd. [M + Na-2H]− 9649.77, found: 9648.32. e) MALDI-TOF MS spectrum of the cross-linked complex degradation products by DNase I, calcd. [M + H]+ 1037.52, found: 1037.57.
Image published in: Yuan L et al. (2013)
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