Larger Image Figure 6. Menthol attenuates α7 nACh receptor calcium signaling.(A) Live cell imaging of cells expressing GCaMP5G. ROI within soma and neurite shown via the organge and red dotted line, respectively. ROI selection is based on co-detection of fBgtx and GCaMP5G as indicated in Fig. 5. Rows top to bottom: cells pre-treated with Control (0.3% ethanol); Menthol (30 µM); α-bungarotoxin (Bgtx) (50 nM). Image frames captured at 6/8/10/12 seconds (s). PNU (10 µM) was applied at 8–10 s. (B) Average fluorescence signal data for soma and neurite ROI imaged for 20 seconds. n = 40 cells, **P>0.01”. Image published in: Ashoor A et al. (2013) Image reproduced on Xenbase with permission of the publisher and the copyright holder. Creative Commons Attribution license Permanent Image Page Printer Friendly View XB-IMG-128931