Figure 6. Uhrf1 exhibits two phases of chromatin binding. (A) XB extract containing sperm nuclei was incubated at 21°C. Replication inhibitors, geminin (80 nM) and roscovitine, were included as shown. Chromatin was isolated at the times indicated and associated proteins detected by Western blotting. Orc1 serves as a loading control. (B) A quantification of the Uhrf1 signal relative to Orc1 loading control is presented.
Image published in: Taylor EM et al. (2013)
© The Author(s) 2013. Creative Commons Attribution license
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