Figure 6. Results of the screen.The 20 active clones identified during the screen were individually injected and analysed by Western blot to demonstrate their abilities to modulate the activities of different signalling pathways as shown in Table 3. Embryos were collected at the indicated stages, processed, and analysed by Western blot to assess the activities of Erk (pErk, panel A), Akt (pAkt, panel B), BMP (pSmad1, panel C), and TGFβ/Nodal (pSmad2, panel D). Control (ctrl) denotes uninjected embryos. Anti-Erk (Erk), anti-Akt (Akt), anti-Smad2 (Smad2) and anti-α-Tubulin (α-Tubulin) were used as loading controls.
Image published in: Zhang S et al. (2013)
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