Fig. 1. Expression screen for direct transcriptional targets of Wnt signaling in neural tissue. (A) X. tropicalis embryos stained for transcripts identified as Wnt targets by RNA-seq. Stage 10.5 embryos show dorsovegetal views with the dorsal lip toward the top. Stage 12 and 15 embryos show dorsal views with anterior toward the top. (B,C) qPCR on 15-25 animal caps treated as indicated on the x-axis. The y-axis shows expression relative to odc. n=4 experiments. (D) Luciferase reporter assays in HEK293 cells treated with or without mouse Fgf. n=3 experiments. Error bars indicate s.e.m. All means were compared by one-way ANOVA followed by Tukey post-hoc analyses (*P<0.05).
Image published in: Young JJ et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| prickle1.S | prickle, prickle1-a, prickle1-b, prickle A, prickle B, Prickle-b, XPk, XPk-1 | X. laevis | Throughout NF stage 10.5 | marginal zone |
| sf3b4.S | spx | X. laevis | Throughout NF stage 10.5 | ectoderm dorsal |
| hnrnpk.S | X. laevis | Throughout NF stage 10.5 | ectoderm dorsal marginal zone | |
| sall1.L | LOC108715370, sal-1, sal1, sall1-a, sall1-b, sall1b, sal-like 1, spalt-like 1, tbs, znf794 | X. laevis | Throughout NF stage 10.5 | dorso-lateral marginal zone ectoderm mesoderm involuted dorsal mesoderm |
| sall4.L | drrs, znf797 | X. laevis | Throughout NF stage 10.5 | ectoderm marginal zone |
| srsf7.L | 9g8, aag3, sfrs7 | X. laevis | Throughout NF stage 10.5 | ectoderm dorso-lateral marginal zone |
| prickle1.S | prickle, prickle1-a, prickle1-b, prickle A, prickle B, Prickle-b, XPk, XPk-1 | X. laevis | Throughout NF stage 12 | neural plate neuroectoderm neural groove circumblastoporal collar |
| sf3b4.S | spx | X. laevis | Throughout NF stage 12 | ectoderm neuroectoderm dorsal |
| hnrnpk.S | X. laevis | Throughout NF stage 12 | circumblastoporal collar | |
| sall1.L | LOC108715370, sal-1, sal1, sall1-a, sall1-b, sall1b, sal-like 1, spalt-like 1, tbs, znf794 | X. laevis | Throughout NF stage 12 | circumblastoporal collar posterior neuroectoderm neural plate |
| sall4.L | drrs, znf797 | X. laevis | Throughout NF stage 12 | neural plate neuroectoderm chordal neural plate circumblastoporal collar |
| srsf7.L | 9g8, aag3, sfrs7 | X. laevis | Throughout NF stage 12 | neural plate neuroectoderm |
| prickle1.S | prickle, prickle1-a, prickle1-b, prickle A, prickle B, Prickle-b, XPk, XPk-1 | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm circumblastoporal collar neural groove |
| sf3b4.S | spx | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm |
| hnrnpk.S | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm neural groove circumblastoporal collar | |
| sall1.L | LOC108715370, sal-1, sal1, sall1-a, sall1-b, sall1b, sal-like 1, spalt-like 1, tbs, znf794 | X. laevis | Throughout NF stage 15 | neural groove posterior neuroectoderm circumblastoporal collar |
| sall4.L | drrs, znf797 | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm neural groove circumblastoporal collar |
| srsf7.L | 9g8, aag3, sfrs7 | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm chordal neural plate |
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