Fig. 4 (Left). Spatial expression of arid5b during Xenopus development. In situ hybridization of whole (A-D, G-L) or transverse fractured embryos (E,F) at the indicated stages of development with antisense probe for arid5b (nucleotide 775 to 1612 of isoform1) (A,B,D,E,H,J,L,I), arid5b arid5b arid5b sens arid5b-slc12a1 arid5b slc12a1 arid5b-slc12a1 arid5b arid5b delta1 delta1 arid5b-slc12a1 st 22 st 25 st 25 st 31 st 25 st 25 st 35/36 st 35/36 st 35/36 st 35/36 st 35/36 st 35/36 G B C D E F H I J K L A delta1 (C,F), slc12a1 (I,K,L) and with control sense probe for arid5b (G). Lateral views with anterior to the right (A-D,G,H,J-L). Transverse section at the level of the proximal pronephric tubule. In I and L, arid5b is revealed in light blue and slc12a1 in purple. Arid5b mRNAs are detected in the anterior pronephric anlage during tailbud stages in a broader domain than delta1. At tadpole stage 35/36, arid5b expression is restricted to the proximal part of the tubule and does not overlap with slc12a1 expression which is specific for the intermediate and the first segment of the distal tubule. Scale bars are 0.3 mm.
Image published in: Le Bouffant R et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| arid5b.S | LOC108696083 | X. laevis | Throughout NF stage 22 to NF stage 25 | pronephric mesenchyme |
| dll1.L | delta-1, Delta-1, delta1, x-delta, X-delta-1, Xdelta-1, Xdelta1, XDelta1 | X. laevis | Throughout NF stage 25 | pronephric mesenchyme optic vesicle neural tube |
| arid5b.S | LOC108696083 | X. laevis | Throughout NF stage 31 to NF stage 35 and 36 | pronephric kidney pronephric nephron pronephric tubule proximal tubule |
| slc12a1.L | Na-K-2Cl cotransporter, NKCC-2, NKCC2, XNKCC2 | X. laevis | Throughout NF stage 35 and 36 | distal tubule |
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