Fig. 1. Dystroglycan depletion affects notochord morphogenesis. (A) Immunodetection of Dg on transverse cryosections from stage 13 to 37 (indicated in the top right of the images). The arrowhead in c indicates a vacuole. (B) Immunoblot of protein extracts from wild-type (WT) embryos or embryos injected with a 5-mispair control morpholino (C-MO) or Dg-MO. (C) Cryosections of embryos co-injected with ras-GFP mRNAs and C-MO (a) or with Dg-MO (b) and then immunostained with antibodies against Dg. In Dg morphants, the Dg staining is lost. (D) The top panels show the embryo phenotypes observed with C-MO or an increasing amount of Dg-MO at stage 32. (a-d) Cryosections treated with Tor70 antibodies showing a dose-dependent effect on the notochord. The arrowhead in c indicates the discontinuity in the notochord sheath. A cell containing Dg-MO (labeled with GFP, arrow and inset in c) escaped from the notochord. (d) At a high amount of Dg-MO, the sheath disappears and the characteristic structure of the notochord is lost. Dotted lines outline the neural tube (NT). (E) Notochord morphogenesis is rescued in Dg morphants through injections of mRNA encoding Dg (Dg-FL mRNA). Dotted lines outline the NT. NC, notochord; S, somite. Scale bars: 25â μm in A,C,Da-d,E; 500â μm in upper panel in D; 10â μm in Dc inset.
Image published in: Buisson N et al. (2014)
Copyright © 2014. Image reproduced with permission of the Publisher.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| dag1.L | alpha-dystroglycan, dystroglycan | X. laevis | Sometime during NF stage 13 to NF stage 37 and 38 | notochord |
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