Figure S7. PN1 promotes anterior development via inhibition of HtrA1-, FGF- and Wnt signals Embryos were animally injected with MOs at the 2-cell stage and mRNAs at the 4-cell stage. (A) Anterior view of early neurula embryos after whole-mount in situ hybridization with Otx2, demarcating the prospective cement gland, forebrain and midbrain. (B) PN1 mRNA (4 ng) causes expansion of Otx2 expression. (C,D) PN1-MO (60 ng) reduces the Otx2 marker, while an equivalent amount of control-MO has no effect. (E,F) HtrA1-MO (20 ng) or 80 pg δXFGFR-4a mRNA restore normal Otx2 expression in PN1 morphant embryos. Indicated phenotypes were observed in B, 12/13; C, 59/62; D, 63/73; E, 22/24; F, 23/29.
Image published in: Acosta H et al. (2015)
Copyright © 2015. Image reproduced with permission of the Publisher.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| otx2.S | otx-2, otx2-a, otx2-b, otxA, Xotx-2, Xotx2 | X. laevis | Throughout NF stage 14 | optic field pre-chordal neural plate |
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