Figure 4. Rescue of early neural development in UV-ventralized embryos. RNA injected per embryo: 20 pg for E1A and RG, 3 ng for p300N, and 150 pg for chordin. In situ hybridization and RT-PCR were performed on embryos at stage 14. A, Rescue of zic-r1 expression by E1A and chordin, but not by RG2 or b-galactosidase (used as a lineage-tracing marker). Note that the expression of zic-r1 overlaps with X-gal staining in E1A or chordin (chd)-injected embryos. B, Rescue of neural markers examined by RT-PCR.
Image published in: Kato Y and He X (1999)
Copyright © 1999. OA ARTICLE, images redisplayed under a Creative Commons license.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| zic1.S | odd-paired-like, opl, Zic-r1, Zic-related-1 | X. laevis | Throughout NF stage 13 | neuroectoderm |
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