Fig. 2 Embryonic expression of ube2d3.2. (now called ube2d4) (A) A polyclonal antibody raised to amino acids 1â147 of ube2d3.2 was used to probe control embryos (uninjected) and embryos expressing FLAG-tagged ube2d3.2(-FLAG). (B) Equal quantities of total embryonic protein from the indicated stages were loaded onto an SDS-PAGE gel and probed with the polyclonal antibody raised against ube2d3.2, upper panel. A duplicate membrane was probed with pre-immune serum from the rabbit used to produce the specific ube2d3.2 antibody, middle panel (both the upper and middle panels were exposed for the same time). Tubulin was used as a loading control lower panel. ( C) Expression pattern of ube2d3.2 mRNA at stages 28 and 35. Panels show lateral views with anterior to left and the right panel is a magnification of the middle panel. AS, antisense probe. The somites (s), eye (e), otic vesicle (o), branchial arches (ba), and pronephric primordium (p) are indicated.
Image published in: Jean S and Moss T (2008)
Copyright © 2008. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| ube2d4.L | HBUCE1, ube2d3.2 | X. laevis | Throughout NF stage 28 | intermediate mesoderm otic vesicle somite presomitic mesoderm pronephric mesenchyme optic vesicle |
| ube2d4.L | HBUCE1, ube2d3.2 | X. laevis | Throughout NF stage 35 and 36 | pronephric kidney otic vesicle somite presomitic mesoderm brain tail pharyngeal region |
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