Fig. 2. BMP pathway activity in HAEC cultures. (A) Western blot with an anti-pSMAD1/5 antibody revealed that in HAEC cultures SMAD1/5 phosphorylation increases during the early regeneration stages, then decreases at the onset of ciliogenesis. Pr, proliferation stage (ALI days 0-7); Po, polarising stage (ALI days 7-15); EC, early ciliogenesis (ALI days 15-30); LC, late ciliogenesis (from ALI day 30). HSP60 (HSPD1) was used as a loading control. (B) In HAECs at Po stage, pSMAD1/5 labelling was mostly restricted to apical layer cells. (C,D) The number of pSMAD1/5-positive cells was slightly increased in the apical layer and dramatically increased in the basal layer following a 2 h treatment with BMP2 (100 ng/ml) at Po stage. Data in C are representative of three independent experiments; error bars indicate s.d. (B,D) Orthogonal views (z-slices) of HAECs.
Image published in: Cibois M et al. (2015)
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