Fig. 1. ADAM19 expression. Quantitative real-time PCR expression of ADAM19 during early Xenopus development. Polyadenylated RNA from 2.5 embryos was used to make cDNA. PCR amplification was performed on a light cycler for 40 cycles. (A) The amplification product from both the control (âRT) and the experimental (+RT) were separated on a 2% agarose gel. (B) The relative abundance of the ADAM19 mRNA was measured by the 2(-δCT) method using stage 10 as a value of 1. Values are not adjusted to a control gene but to identical number of embryos. The CT values for each reaction are given below the graph. No CT values were obtained for the âRT control of each stage. ADAM19 mRNA is present as a maternal transcript at stage 2. Zygotic transcription starts during gastrulation and increases during neurulation (stage 16) continuing into early tailbud stage (stage 21).
Image published in: Neuner R et al. (2009)
Copyright © 2009. Image reproduced with permission of the Publisher, Elsevier B. V.
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