Figure 4. Use of explants to determine the role of proteins in tissue repulsion. The left side of the figure shows a wholemount in situ hybridization of a bisected Xenopus embryo with a probe for ephrinB1 expression; showing expression in the involuting marginal zone. The right side is a schematic depicting the BCR/mesoderm repulsion assay. Four cell stage embryos are injected with either mRNA or MOs or both, along with fluorescent dextran as a tracer. The mesoderm (involuting marginal zone) is removed and placed upon the blastocoel roof (BCR) explant from an uninjected embryo. Light and epifluorescence microscopy allows for visualization of whether the two explants mix or repulse one another
Image published in: Hwang YS and Daar IO (2017)
Copyright © 2017. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| efnb1.L | cfnd, cfns, efl3, elk-l, ephrinB1, eplg2, lerk2 | X. laevis | Throughout NF stage 11 | involuting marginal zone |
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