Fig. 4. Prdm13 loss of function leads to a decrease in glycinergic but not GABAergic-amacrine cells. a Whole-mount in situ hybridization analysis of prdm13, glyt1, gad1 and vglut1 expression on stage 39/40 embryos injected with prdm13-M0 or control-MO. Lateral views of the head and transversal sections of the retinas are shown. The number of analysed embryos and the percentage of embryos with represented phenotypes are indicated in each panel. b Stage 39/40 sections following GABA or Glycine-immunostaining on control-MO and prdm13-MO injected embryos. Arrows point to Glycine-positive cells. c Quantification of the average number of GABA- or Glycine-positive cells per section. Number of analysed sections is indicated in each bar. Data are presented as mean ± SEM. p < 0.001 (***) (Mann-Whitney test). Scale bars represent 200 μm (whole mount), 100 μm (sections)
Image published in: Bessodes N et al. (2017)
© The Author(s). Creative Commons Attribution license
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| slc17a7.L | bnpi, vglut1, Xvglut, Xvglut1 | X. laevis | Sometime during NF stage 39 to NF stage 40 | retina retinal neural layer eye brain midbrain hindbrain otic vesicle cranial placode |
| prdm13.L | X. laevis | Throughout NF stage 40 | retina hindbrain | |
| slc6a9.L | glyt1, glyt1b, xGlyT1 | X. laevis | Throughout NF stage 40 | retina retinal neural layer eye |
| gad1.L | cpsq1, gad1.1, gad67 | X. laevis | Throughout NF stage 40 | retina retinal neural layer brain |
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