Fig. 2. The Xenopus animal cap explant procedure. SyntheticmRNAencoding a potential regulator of cardiac development is injected into the fertilized Xenopus egg at the one cell stage. When the embryo has reached the blastula stage, the animal cap (ectodermal tissue overlying the blastocoel cavity) is carefully removed using fine forceps and it placed into explant culture. After an appropriate period in culture, the cap tissue and is subjected to appropriate assays for gene expression. Reprinted from [68] with permission from Elsevier.
Image published in: Warkman AS and Krieg PA (2007)
Copyright © 2007. Image reproduced with permission of the Publisher.
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